To assist clearing, hairs should not be more than 5 mm long, and skin scales, crusts, and nail snips should not be more than 2 mm across. First, wear the groves. Capillary action will pull the dye across the slide to stain the specimen. Take a small drop of the sample you want to view and place it on the center of the microscope slide. See different staining types and preparation techniques for more information. 2. Start with a clean microscope slide and cover slip. Take photographs of the specimen and make special note of any abnormal or unusual findings. Cover the specimen with a cover slip, being careful to avoid air bubbles. Study Area Mix a drop of stain into the specimen preparation with a toothpick. To make one, place a drop of the sample in the middle of a clean slide and lower a cover slip gently over the drop at an angle, with one edge touching the slide first (See Figure 1, below). MicroscopeMaster website is for educational purposes only. For example, scientists and health professionals use Methylene Blue, a slightly alkaline stain, to reveal the presence of deoxyribonucleic acid, more commonly known as DNA. This will draw out some water. Id]Hvm>s&;`1TtH View under 400X magnification immediately, otherwise the culture will dry and you will not see anything move. Label the slide with the name of the specimen, the date and your initials. Repeat this process two more times for a total of four coats. Set up your microscope at your workstation. a cross-section of a colorless plant stem), it may be difficult to see when looking through a microscope. HU]k0}7?Gi,,>4Jvd!!sJ`KdZBs=Bj.AAm&TuHXu0}&W4O4!XT;ka.M>&M.h]Sj3u0^Da(@Ga|kUsQ(\`M(- KP..'/~;4|5c84zrCMUb6I4#.AF'ht7J#EW*{f>1BS5(2t}R'1Dhcek*`T vAC}Qz`pr9 ^Ai~X+RBM{Cj ^m]UISO6{5_^K(@b JZhM[]X3Tt*^oJ4SQfpU:>KRBYqumS2Dn5.]S6otgX&WtO@A4]g^ZI|hVd These more expensive microscope slides can be used without a cover.if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[300,250],'microscopemaster_com-medrectangle-4','ezslot_5',114,'0','0'])};__ez_fad_position('div-gpt-ad-microscopemaster_com-medrectangle-4-0'); Some manufacturers produce plastic chambers with a predetermined number of slides with covers. take the utmost precaution and care when performing a microscope 72 0 obj <> endobj Place the coverslip over the drop of urine. Wladimir Bulgar / Science Photo Library / Getty Images. 5 Why do you need to prepare a wet mount? If too dry, add a drop of water beside the cover slip. Iodine is one of the more commonly available stains and is used to identify starch in a variety of samples. All corners are a sharp 90-degrees and, along with a rough outer edge, can cause minor finger cuts if not handled with care. These are dry mount, wet mount, and prepared mount. This article has been viewed 86,883 times. Otherwise, seal around the edges of the coverslip with a thin film of clear nail polish (fig. Place the specimen on the slide and put a frop of water on it to make it stick. Place the slide on the microscope stage and lower the stage until the specimen comes into focus. Supplies 1. Make sure to avoid any air bubbles. For example, use a concave slide if youre preparing a curled flower petal that rolls to one side or the other. Number 2 covers, .17-.25 mm, are designed for general purpose. To one sample area of the slide, add a drop of KOH Coverslip both sample areas and examine under a microscope. Less frequent characteristics include rectangular shapes, alternative materials such as quartz and certain types of plastic, etched lines or grids and additional thicknesses.if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[336,280],'microscopemaster_com-large-leaderboard-2','ezslot_12',123,'0','0'])};__ez_fad_position('div-gpt-ad-microscopemaster_com-large-leaderboard-2-0'); If not creating a permanent slide with glue and/or sealant, cover slips can be removed and sterilized for multiple reuses. Add stains or dyes to the specimen as necessary to enhance visibility. Apply gentle heat by passing the slide over a Bunsen burner 3-4 times in . How to Prepare Microscope Slides. If the stool specimen is still somewhat solid, add a drop or two of saline to the specimen and mix. evaporation. if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[728,90],'microscopemaster_com-medrectangle-3','ezslot_4',148,'0','0'])};__ez_fad_position('div-gpt-ad-microscopemaster_com-medrectangle-3-0'); The most basic of all microscope slides is a flat rectangular piece of soda lime glass, borosilicate cover glass or plastic, with ground edges. 89 0 obj <>/Filter/FlateDecode/ID[<805C463A0618AA4683A03A421F488943><2F25A7CD4100F14C97045E7430F981BF>]/Index[72 41]/Info 71 0 R/Length 91/Prev 216683/Root 73 0 R/Size 113/Type/XRef/W[1 3 1]>>stream Biological waste container and bag 4. Additional variations to microscope slides include: if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[580,400],'microscopemaster_com-banner-1','ezslot_7',111,'0','0'])};__ez_fad_position('div-gpt-ad-microscopemaster_com-banner-1-0'); Almost always made of borosilicate or silicate glass, cover slips hold samples in place and protect them from inadvertent movement and contamination. Made of glass or plastic, slides are approximately 1x3 inches and between 1mm-1.2 mm thick. A thicker slide can be created with two drops, but only with the blood of mammals as the erythrocytes lack a nucleus allowing cells to be amassed in multiple layers. _a#Pi\gm2B=fo[N t!u=Qol~pC?G?2?Hkx These solutions may be used to increase contrast in wet or dry mounts. Used for aquatic samples, living organisms and natural observations, wet mounts suspend specimens in fluids such as water, brine, glycerin and immersion oil. Do not go to oil, you may crack the coverslip. In this method, a drop of culture is placed on a coverslip that is encircled with petroleum jelly (or any other sticky material). Usually, slides can be rinsed and reused, but using nail polish means the slides must be cleaned with polish remover before reuse. Deltaproteobacteria is a large group (Class) of Gram-negative bacteria within the Phylum Proteobacteria. A wet mount is made by placing a fluid solution on a slide, suspending a specimen in a solution, and then covering the specimen and the solution with a cover slide. If viewing a sample not already in the liquid, use tweezers to position the specimen within the drop. As the liquid evaporates from the edges of the slide, living samples may die. Whether you mount samples, pipette into wells or use prepared slides, microscope slides offer a vehicle to contain a specimen while observing under magnification. Saving time and money, electrostatic charged slides are a popular choice for researchers of histology, Suited for high-resolution microscopy and. The slide may be already prepared, even if it is not stained. Standard glass microscope slide Pipette Specimen sample Water Slide coverslip Tweezers Paper towel Q-tip The first step is to use the Q-tip to swab your sample and apply it to the center of the microscope slide. Apply a thin layer of clear nail polish over the entire surface of the coverslip. Make a wet-mount preparation of the Staphylococcus culture, following the same procedures just described. Remove excess water from slide using filter paper on side of coverslip. Use a sweeping motion to ensure no air bubbles are trapped in the polish. If your wet-mounted slide specimen is pale or colorless (e.g. BW cJPeA[EW})E&umCm wp`UfNzuOf@0w.^oON@EmaUfH5M9,BwwY/*Nn? Place a cover slip over the specimen. Always handle the slides with care, as they can easily be shattered or scratched. Do not tilt the slide in your other hand while you are doing this. 3 What is the advantage of using a wet mount preparation? Bess Ruff is a Geography PhD student at Florida State University. To prepare a wet mount using a flat slide or a depression slide: Some living organisms move too quickly to be observed in a wet mount. Staining provides contrast through color that reveals structural details undetected in other slide preparations. Hour: Slip Covers C. PipetD. We and our partners use cookies to Store and/or access information on a device. MicroscopeMaster is not liable for your results or any Yw3 ~00M`0dg*?AJ)Xi>! ( - Use your tweezers and remove a single layer from the inner side of the onion section. Smear slides require two or more flat, plain slides, cover slips, pipette and tissue paper: if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[580,400],'microscopemaster_com-large-mobile-banner-1','ezslot_15',140,'0','0'])};__ez_fad_position('div-gpt-ad-microscopemaster_com-large-mobile-banner-1-0'); Ideally, smears should dry naturally in an environment of moderate, steady temperature. Ur30$Fu0022*%%^Q78*yrvs/, C Collect your specimen: choose a thin, flat specimen like a plant leaf, insect wing, or single-cell organism. hb```N{>c`0pbj\>1A~: Be sure to adjust the focus in order to get a clear view. Grasp the cover slip by the outer edges. 18 terms. Place one drop of the liquid specimen in the medicine dropper on top of the slide. We use cookies to make wikiHow great. Apply another thin layer of polish to the top side of the coverslip. To use one of these stains: Many common foods and objects make fascinating subjects for slides. \8)SBI Cover slips are very thin, transparent pieces of glass or, more commonly, plastic. In a section mount, an extremely thin cross-section of a specimen is used. See more details >, Eosin Y is an acidic stain which stains pink for alkaline cells (cytoplasm, for example). depth very shallow. Gently press down on the coverslip with a pencil or a slide press to secure it in place. Microscope slides are pieces of transparent glass or plastic that support a sample so that they can be viewed using a light microscope. Step 1 Use a flat glass slide to prepare a wet mount. vuC.f0w;RL`\{ [ 3k Put a drop of stain on an outer edge of your cover slide. * Staining Bacteria *. Mix well on the slide. 3.2, step 3). Rounded safety corners to prevent accidental cuts as well as beveled edges with clipped corners ideal for blood samples are options available for both generic and frosted slides. Select a microscope slide and place it flat on a clean surface. It may be necessary to use a knife or razor blade to shave a section of the sample. is easily solved by preparing a vaseline chamber. Observe preparation under the 40X objective lens. What are the three steps to making a wet mount slide? Place a sample on the slide. Wet mount slides are used to view living organisms, as well as liquid substances of all kinds. 1 What are the steps to make a wet mount? Wet Mount: Place a drop of fluid in the center of the slide. If desired, you can also add a layer of mulch to the top of the mound. , Number 1 covers are .13-.17mm thick. A drop of the solution is added to the liquid drop before applying the coverslip. Examples of appropriate subjects include: Dr. Helmenstine holds a Ph.D. in biomedical sciences and is a science writer, educator, and consultant. hb``g``j````6; Place the cover slip over the sample, making sure the edges align. Saving time and money, electrostatic charged slides are a popular choice for researchers of histology, cytology and pathology. WoB8%"Z}[ qiXx1 i}=6KXl?|9E e: HU0}7QZ2eA0ZRX@}Hn;rvBSS|9 *W 9}"4!C pr9h? :x_-aQxdD8 O0&~&x"H6!lj[=CF^`8 &# l:%#:0eZaKcQ*Pv?3^=w/RfZ2k)2mDwB First I used paper to represent the specimen. Read more here. In a wet mount, a drop of water is used to suspend the . Select the specimen you wish to examine, or prepare a sample if needed. Place a drop of the urine sample on a clean glass slide. This is the "wet" part of the wet mount. In this case, 84% of readers who voted found the article helpful, earning it our reader-approved status. ", http://www.microscopemaster.com/microscope-slides.html, https://learning-center.homesciencetools.com/article/microscope-slide-making-ideas/, https://www.carolina.com/teacher-resources/Interactive/make-microscope-slides-for-science-fair-projects/tr10768.tr, https://www.youtube.com/watch?v=YU0DYrU_0FM&feature=youtu.be&t=1m40s, https://www.youtube.com/watch?v=YU0DYrU_0FM&feature=youtu.be&t=1m51s, https://www.youtube.com/watch?v=YU0DYrU_0FM&feature=youtu.be&t=1m54s, https://www.youtube.com/watch?v=YU0DYrU_0FM&feature=youtu.be&t=2m11s, preparar un portaobjetos para microscopio. One way to retard evaporation is to use a toothpick to coat the edges of the coverslip with a thin rim of petroleum jelly before dropping the coverslip over the sample. Be certain the nail polish is completely dry before examining the slide under the microscope. Scrape inside of cheek with toothpick (firmly but gently). Microscopic I I The McGraw-Hill, Laboratory Manual and Microbiology Morphology of Companies, 2003, Workbook in Microbiology, Microorganisms 7/e, Stretch Coach Compartment Syndrome Treatment, Fluxactive Complete Prostate Wellness Formula, Candle Jar Microbiology - Microbiology Action, Streaking An Agar Plate - Microbiology Action. Organisms such as protozoa may only live 30 minutes under a wet mount slide; applying petroleum jelly to the outer edges of the cover slip creates a seal that may extend the life of the slide up to a few days. Carefully place a clean cover glass (see Experiment 3.1, procedure 1) over the drop, trying to avoid bubble formation (fig. A wet mount slide is the most common type of slide preparation for microscope work. Suited for high-resolution microscopy and oil immersion preparations, Number 1 covers are .13-.17mm thick. How to Grow Table Salt or Sodium Chloride Crystals, Surface Tension - Definition and Experiments, How to Do Chromatography with Candy and Coffee Filters, Ph.D., Biomedical Sciences, University of Tennessee at Knoxville, B.A., Physics and Mathematics, Hastings College. MicroscopeMaster website is for educational purposes only. Then place on edge of the cover slip over the sample and carefully lower the cover slip into place using a toothpick or equivalent. Microscopy Lab Quiz. Put down the medicine dropper. In a wet mount, a drop of water is used to suspend the specimen between the slide and cover slip. Get a slide and clean it with a paper towel and water. To view the purposes they believe they have legitimate interest for, or to object to this data processing use the vendor list link below. By signing up you are agreeing to receive emails according to our privacy policy. These steps generally dictate the layout and workflow in large, specialist histopathology laboratories where hundreds of specimens are handled every day. Great job. Place the cover slip on top of the slide, making sure the edges of the cover slip match up with the edges of the slide. 0 Make sure there are no air bubbles trapped underneath. How to Prepare a Wet Mount of Cheek Cells Before starting, it's always important to ensure that the working surface is clean and that you are wearing a pair of clean gloves to avoid contamination. If examining a plant cell, tap water can be used. Hanging drop preparation is a special type of wet mount in which a drop of medium containing the organisms is placed on a microscope slide, often used in dark illumination to observe the motility of bacteria. FhKR7 Three of the most common methods are wet mounts, dry mounts, and smears. When placing a cover slip, there is no adhesive needed. 5^ffk_ MyyGQ%rHKI &Qf9:*;iJWjS9t%:CZ OF`:"r(J81*JrLJ:De^c(]*zF;Q*9'j'QYtR!M~9+E@/*E#Qj1PXWNodlNKx>x .z=X=a!~-O4898&}"i{=^f5^.\ I_)gr@[q`3FV:wtu~30 ;g Carefully place a clean cover glass (see Experiment 3.1, procedure 1) over the drop, trying to avoid bubble formation (fig. Stains are used to help identify different types of cells using light microscopes. Place a sample on the slide. Morello-Mizer-Granato: I I. Wet mounts differ from. Next, add a few more drops of liquid until the specimen is covered. The material onthis page is not medical advice and is not to be used Do not use stain if viewing photosynthetic cells (which already appear green due to chlorophyll), or living organisms, such as protozoans in pond water (stains will kill them). Directions: Arrange the sequence of the steps on preparing a wet mount by writing letters M O U N T inside the box. for diagnosis or treatment. New Zealand is about 2,000 kilometres (1,200 mi) east . By using our site, you agree to our. 1.Obtain a clean slide and cover slip. You can prepare home-cooked meals with the 2-burner cooktop, 12V refrigerator and single bowl stainless steel sink, and then enjoy them at the front booth dinette which can transform into a 54 x 74 bed. 3. endstream endobj 226 0 obj <. Preparing a Wet Mount Introduction The Wet Mount is a procedure performed in the laboratory to observe motile organisms. Create a detailed storyboard, which will include all of the scenes, shots, and transitions that will be necessary for the film. As a small thank you, wed like to offer you a $30 gift card (valid at GoNift.com). Wet mounts should ideally have no air bubbles because beginners may have trouble distinguishing the bubbles from the specimen when looking under the microscope. Multiple methods of preparation allow for advanced viewing of inorganic and organic objects.. %PDF-1.5 % M for the first step followed by O for the second step and U, N, and T for the next steps. Gently tap the cover slip so that the water spreads evenly over the tissue and the edges of the cover slip. Read further about Blood Smears - process and technique, possible presence of artifacts. Remove excess water with the paper towel. observation. Make sure to spread it evenly across the surface and to avoid overlapping the edges. - If the layer you removed is not translucent to light, then try again. Place the tissue on the slide and add a few drops of water. % of people told us that this article helped them. %%EOF 9 years ago Observe and draw the prepared wet mount containing the onion cells at the frequency: 10X 50X 100X 3. Gather the necessary supplies, including prepared microscopic slides, a microscope, fresh clean slides and cover slips, immersion oil, dyes and stains, and a pipette. Using a microscope, observe the wet mount slide. (5[- Dry Mount: The dry mount is the most basic technique: simply position a thinly sliced section on the center of the slide and place a cover slip over the sample. contrast. 1. 0 A cover slip may be used to keep the specimen in place and to help protect the objective lens. Clean a slide and cover slipping using lens paper. Mulch will help retain moisture and help to suppress weeds. Use the edge of this slide to touch the drop. Place the slide on a flat surface and carefully place one drop of water on the slide. Gather your materials: microscope slide, cover slip, watch glass or petri dish, water, and a pair of tweezers. Helmenstine, Anne Marie, Ph.D. "How to Prepare Microscope Slides." Once the iodine or methylene blue has drawn all the way under the slide cover, the specimen is fully dyed. Methylene Blue is an alkaline stain useful in identifying acidic cell nuclei and DNA in animal, bacteria or blood samples. Glycogen will show as red. Some of the specimens (e.g., a strand of hair or an insect wing) are thin and translucent on their own, and will not need to be sliced with a razor blade. 2.Using a transfer pipette, obtain a drop of specimen and place onto the center of the slide. Spread it out using a glass insertion needle. Practice this until you get used to it. When it is complete, look at it under the microscope. Once the slide is heat fixed, it can then be stained. Can you use a stain on a wet mount slide? Place the slide on a piece of paper towel and use a dropper to add 2-3 drops of water to the slide. Approved. field microscopy, differential interference Film, using the storyboard to guide you. Each type of preparation method is used for mounting different types of cells. Wet mounts are used for living samples, transparent liquids, and aquatic samples. Place a cover slip over the sample, taking care not to trap air bubbles. Turn on the microscope and adjust the focus until the specimen is clear. You may need to add another drop to ensure complete coverage. Put the slide on the microscope: use the tweezers to carefully pick up the slide with the cover slip on top of the specimen and place it on the microscope stage. In a wet mount, a drop of water is used to suspend the specimen between the slide and cover slip. If you continue to use this site we will assume that you are happy with it. Without the cover in place, surface tension would cause the droplet to bunch up in a dome. Have the patient lie down on an exam table with their feet in stirrups. In order to heat fix a bacterial smear, it is necessary to first let the bacterial sample air dry. Be sure to set up proper camera angles and lighting, capture good sound, and have a plan for filming each scene. Figure 1: A diagnostic section being prepared with a cryostat microtome. The "mount" is simply the way in which a specimen is placed on the slide. The 2. It should be about 2 cm2. 2. Prepare a second, similar slide using potassium hydroxide. Continue with Recommended Cookies. Yes your quite right. Place the coverslip on top of the sample. Then place on edge of the cover slip over the sample and carefully lower the cover slip into place using a toothpick or equivalent. Follow these directions. Using a dampened cotton swab, apply pressure to the cover slip to remove any air bubbles. We have tried a new approach to routine stool microscopy by using a combination of methylene blue and glycerol in wet mount preparation of fresh faecal samples for the demonstration of medically important intestinal parasites. Using a pipette bulb, aspirate a small amount of the Proteus culture with a capillary pipette and place a small drop on a clean microscope slide (fig. this page, its accuracy cannot be guaranteed.Scientific understanding Simply take a single The first step in making your own slides is to choose the type of mount you want. Section mounts are suitable for useful for a wide variety of samples such as fruit, vegetables and other solids that can be cut into small slices. make a film. Number 2 covers, .17-.25 mm, are designed for general purpose. You should avoid thick formulas when sealing coverslips. Use your free hand to carefully pick up a cover slip (they are extremely fragile). To prepare a wet mount using a flat slide or a depression slide: Place a drop of fluid in the middle of the slide (e.g., water, glycerin, immersion oil, or a liquid sample). You should keep the slide as horizontal and steady as possible, and place it on the viewing tray of your microscope. Helmenstine, Anne Marie, Ph.D. (2020, August 28). The liquid used depends on the type of cell being viewed. Obtain as thin a sample of the specimen as possible and place the sample on the water Step 3 to preparing a wet mount slide. Place a coverslip on top of the slide and press down gently. Next is the liquid sample. How to Prepare a Wet Mount Specimen Step One - Obtain a clean microscope slide. Why would use a wet mount? ** Be sure to Slowly lower the coverslip, avoiding air bubbles. This is usually done after you have already examined the wet specimen in a slide without staining it. A variety of methods exist for staining microscope slides, including non-vital or in vitro stains of non-living cells and vital or in vivo stains of living tissue. The slide should be clean and free of dust or other fine particles. Model: ADC H3-8FP: Hyperion 3 3003 Aluminum, NSF approved cookware. Use a flat glass slide to prepare a wet mount. Step-by-step video and audio instructions on how to prepare a wet mount specimen of eukaryotic animal cells; specifically Human epithelial cells from the inside of the cheek. Betaproteobacteria is a heterogeneous group in the phylum Proteobacteria whose members can be found in a range of habitats from wastewater and hot springs to the Antarctic. H_K01ocn* Ho0#G{vb'>:$nEaCFM [9?7tn#N-0R*eViH2 URA.Elqeix ng#?x Rf`nLd0PI$0Hka0 7 Water E. Specimen ( It is the middle of winter I couldn't get a leaf so I used a piece of paper). 3D Printed Futuristic Space Age Wall Clock. Do not tap or press on the cover slip once it is in place. Sterile microscope slides 5. Thanks for the criticism. Using a pipette, place a drop of water on the specimen. Ideally, two smears can be prepared on one slide, of which one can be stained with iodine. Tamang sagot sa tanong: Ons: Arrange the sequence of the steps on preparing a wet mount by writing letters MOUNT inside the box. A vaginal wet mount, also called a wet prep or vaginal smear, is a test done to find the cause of vaginitis, an inflammation of the vagina. Concave microscope slides contain one or more surface depressions ideal for liquid solutions and larger specimens. Enter a Melbet promo code and get a generous bonus, An Insight into Coupons and a Secret Bonus, Organic Hacks to Tweak Audio Recording for Videos Production, Bring Back Life to Your Graphic Images- Used Best Graphic Design Software, New Google Update and Future of Interstitial Ads. The method used to prepare a slide depends on the nature of the specimen. You will be using two main types of slides, 1) the common flat glass slide, and 2) the depression or well slides. Place a cover slip onto the microscope slide so that it covers the thin film of liquid culture. Collect a urine sample in a sterile container and label it. The article, "Thanks a lot. Draw what you see and make %en0h;|(pK,E6Q t^;~!%OA E! Unless the specimen is large and fragile enough Model: MTY 543-392: 504-543-392 Sold by: 1 Each Equivalent model numbers: MTY543392 MTY 543-392 MTY 543392 MTY 543-392 504-543-392 Emulsify the specimen in a drop of 20% KOH on a glass slide with the help of inoculating loop. Then time to place the prepared slide on the microscope bench. This is the wet part of the wet mount. Start by selecting a thin bottle of clear nail polish. x*'89wI:: Place a cover slip at a 45 degree angle over the spicimen with a toothpick under it. Each slip is about. In these circumstances, adding ground pieces of cover glass to the water before the slip layer will create added space and chemicals or strands of cotton can be added to slow the movement of paramecium, amoeba and ciliates. Wet mount means placing a sample in drop of water on slide and then covering it with cover slip. Filled calibrated wells or flasks are viewed quickly without preparing or clipping individual slides to the microscope stage, making this especially useful in sediment studies, such as urine analysis; in addition, some tray designs can be placed in an incubator or refrigerator, allowing for the study of cultured samples. 2nd I should have used a smaller piece of paper to represent a specimen. Your doctor will usually order the test if you're. Dry mount slides can consist of a sample placed on a slide or else a sample covered with a coverslip. This method will help prevent air bubbles from being trapped under the cover slip. Mount the specimen on a concave slide if youre concerned that the specimen will roll or slide off the flat slide. Lower the cover slowly, avoiding air bubbles. Place a drop of water on the center of the slide Step 2 to preparing a wet mount slide. Begin the microscopic review of the slide by examining the saline preparation. B) 1. This helps in better observation. Place a small drop of a liquid sample onto the slide. A wet mount requires a liquid, tweezers, pipette and paper towels.
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